acute monocytic leukemia cell line  (DSMZ)

Journal: European Journal of Medical Research

Article Title: Mechanism of ultrasound-guided renal parenchymal injection of MSCs for the treatment of chronic kidney disease: down-regulated SGK1 promotes M2 macrophage polarization

doi: 10.1186/s40001-025-03724-8

Figure Lengend Snippet: A – F THP-1-derived macrophages were transfected with blank overexpression vectors (Co-culture + OE-NC) or SGK1 overexpression vectors (Co-culture + OE-SGK1), and co-cultured with MSCs for 12 h: Fluorescence intensity analysis of CD68, iNOS, and CD163 in macrophages. G–J The supernatant levels of pro-inflammatory cytokines IL-1β, IL-10, MCP-1, and TNF-α in cell supernatant. ns p > 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Human umbilical cord MSCs (MEISEN, CTCC-159-HUM), human acute monocytic leukemia cell line THP-1 (MEISEN, CTCC-001-0044) and human renal tubular epithelial cell line HK-2 cells (ATCC, CRL-2190) were cultured in DMEM (Gibco, 11,965–092) with 10% fetal bovine serum (FBS; Gibco, 10,099–141) and 1% penicillin–streptomycin (Gibco, 15,140-122).

Techniques: Derivative Assay, Transfection, Over Expression, Co-Culture Assay, Cell Culture, Fluorescence

Journal: Nature Communications

Article Title: Extracellular vesicles derived EBV tegument protein BRRF2 suppresses cGAS phase separation to promote anti-viral innate immune evasion

doi: 10.1038/s41467-025-64037-2

Figure Lengend Snippet: A The box plot shows the differences in the immune and stromal cell compositions between the two groups of tumor samples with different BRRF2 expression levels based on the RNA-seq data. p values were determined via an unpaired two-side t test. Center line, median (50th percentile); box bounds, 25 and 75th percentiles (interquartile range, IQR); whiskers, extend from the bounds to the smallest or largest value within 1.5 × IQR of the bound; minima and maxima, the end points of the whiskers. B Representative images of panCK (green), BRRF2 (yellow) and CD68 (orange) staining in NPC patients’ tumor tissues. Scale bars, 5 μm. C The average distance from BRRF2 + or BRRF2 - tumor cells to CD68 + cells was measured from the IHC images. Each dot represents a patient’s tumor tissue. The experiment was performed as three independent biological replicates. ** p < 0.01 according to a two-sided paired t test. D HPMs and THP1 cells were incubated with HEK293T-induced EVs carrying mCherry-BRRF2 for 4 h, and mCherry-BRRF2 was internalized into the cells. E HPMs and THP1 cells were incubated with HEK293T-induced EVs carrying mCherry-BRRF2 for 4 h. mCherry signals were detected by flow cytometry. The median fluorescence intensity (MFI) is shown. (Means ± SEMs of n = 3 experiments. **** p < 0.0001 according to a two-sided unpaired t test). F EVs from the HEK293T-Flag-BRRF2 cell line inhibited cGAS-STING activation induced by HT-DNA in THP1 and HPM cells. THP1 cells and HPMs were incubated with EVs from the HEK293T-Flag-BRRF2 cell line for 2 h and then transfected with 2 μg of HT-DNA for 6 h. Cell lysates were collected for immunoblotting with the indicated antibodies. The experiment was performed as three independent biological replicates. Representative images was shown. Source data are provided as a file. G After an incubation with EVs and transfection with HT-DNA, total mRNAs were extracted from THP1 cells for a qPCR analysis of the expression of the IFNβ, RANTES and IL6 mRNAs. The error bars are derived from triplicate experiments. The data are plotted as the means ± SEMs. ns indicates not significant. **** p < 0.0001 according to two-way ANOVA followed by Sidak’s multiple comparisons test.

Article Snippet: The human acute monocytic leukemia cell line THP1 (ATCC, #TIB202), the murine breast cancer cell line EMT6 (ATCC, #CRL-2755), and the human NPC cell lines HONE1, CNE2, HK1 (provided from Dr. Sai-Wah Tsao, University of Hong Kong), and Raji (ATCC, #CCL-86), and latent EBV-infected CNE2 (EBV + CNE2) cells were cultured with RPMI-1640 containing 10% fetal bovine serum at 37 °C with 5% CO 2 .

Techniques: Expressing, RNA Sequencing, Staining, Incubation, Flow Cytometry, Fluorescence, Activation Assay, Transfection, Western Blot, Derivative Assay